biotin labelled crna (Thermo Fisher)

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Thermo Fisher biotin labelled crna
Biotin Labelled Crna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotin+labeled+crna/D(%2B)-BIOTIN/pm39702367-112-15-33
Average 99 stars, based on 1 article reviews

biotin labelled crna - by Bioz Stars, 2026-10

99/100 stars

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Article Title: Matrix Metalloproteinase–7 Coordinates Airway Epithelial Injury Response and Differentiation of Ciliated Cells
Article Snippet: Biotin-labeled cRNA was hybridized to Affymetrix Mouse Genome 430A 2.0 oligonucleotide microarrays (Affymetrix, Santa Clara, CA) at the Center for Array Technology of the University of Washington.

Article Title: Long Noncoding RNA TRIBAL Links the 8q24.13 Locus to Hepatic Lipid Metabolism and Coronary Artery Disease
Article Snippet: Biotin-labeled cRNA was hybridized on Affymetrix Human Gene 2.0 ST Arrays (Affymetrix) and results were analyzed with the Transcriptome Analysis Console (Affymetrix).

Article Title: Medicago truncatula as a Model for Dicot Cell Wall Development
Article Snippet: Fifteen micrograms of biotin-labeled cRNA, fragmented as suggested by Affymetrix, was hybridized to the GeneChip® Medicago Genome Array.

Labeling:

Article Title: Use of Genome-Wide Expression Data to Mine the “Gray Zone” of GWA Studies Leads to Novel Candidate Obesity Genes
Article Snippet: Two micrograms of total RNA were treated according to conventional Affymetrix eukaryotic RNA labeling protocols (Affymetrix, Santa Clara, CA). .. Fifteen micrograms of biotin labeled cRNA was fragmented according to Affymetrix eukaryotic sample protocol. .. Hybridization, staining and washing of the Affymetrix U133 Plus 2.0 chips were performed using the Affymetrics Fluidics Station 450 and Hybridization oven 640 under standard conditions.

Article Title: Inflammatory changes parallel the early stages of Alzheimer disease
Article Snippet: In vitro transcription (IVT) reactions were carried out using the Enzo High Yield RNA transcript labeling kit (Affymetrix and Enzo Diagnostics). cRNA was phenol-chloroform extracted on Phase-Lock Gels (Eppendorf), ethanol (100%) precipitated, purified using RNA easy spin columns (Qiagen) and fragmented by heating the samples to 94°C in fragmentation buffer. .. Biotin labeled cRNA was hybridized to the GeneChips for 16 hours, washed on a fluidics station (Affymetrix). .. Probe arrays were scanned on a Hewlett Packard Genearray Scanner at 540nm.

Article Title: Expression of GFP under the control of the RNA helicase VASA permits fluorescence-activated cell sorting isolation of human primordial germ cells.
Article Snippet: .. Following appropriate cleanup of the cRNA (IVT cRNA cleanup spin column as supplied with Affymetrix gene chips), biotin labeled cRNA is quantified and fragmented by metal-induced hydrolysis according to manufacturers protocol (Affymetrix, Buckinghamshire, U.K., http:// www.affymetrix.com). ..

In Vitro:

Article Title: Functionally conserved effects of rapamycin exposure on zebrafish.
Article Snippet: .. Total RNA converted to cDNA was in vitro transcribed in the presence of biotinylated nucleotides to produce biotin-labeled cRNA (antisense RNA, 3' IVT Express kit; Affymetrix, Santa Clara, CA, USA). .. Fragmented cRNA (Ambion; Thermo Fisher Scientific, Inc.) was hybridized to an Affymetrix GeneChip Zebrafish (Affymetrix, Inc., Santa Clara, CA, USA) according to the manufacturer's instructions.

Microarray:

Article Title: Gene expression shift towards normal B cells, decreased proliferative capacity and distinct surface receptors characterize leukemic blasts persisting during induction therapy in childhood acute lymphoblastic leukemia.
Article Snippet: For oligonucleotide microarray hybridization, each RNA sample was linearly amplified by two rounds of in vitro transcription and labeled with biotin using the MessageAmp aRNA Kit (Ambion, Austin, TX, USA) and biotin-11-CTP and -16-UTP (Perkin-Elmer, Boston, MA, USA). .. Biotin-labeled cRNA was hybridized to Affymetrix GeneChip HG U133A chips (Affymetrix, Santa Clara, CA, USA) as described previously.21 Verification of the microarray data for selected genes was performed by real-time reverse transcription-PCR (Supplementary Information and Supplementary Figure S1). ..

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